Induced changes in biochemical parameters of the molluscan tissues non - infected using two potent plants molluscicdes

The effect of Capparis spinosa [C. spinosa] and Acacia arabica [A. arabica] dry powder as plant molluscicide on some glycolytic and gluconeogenic enzymes on snail tissues was investigated. Lactate dehydrogenase [LDH], pyruvate kinase [PK], hexokinase [HK], phosphofructokinase [PFK], glucose phosphate isomerase [GPI] as important glycolytic enzymes were markedly manipulated by both plants when measured one day and one week post-treatment. On the other hand, glucose-6-phosphatase [G-6-Pase], fructose 1.6 diphosphatase [FDpase], phosphoenolpyruvate carboxykinase [PEPCK] as gluconeogenic enzymes were significantly affected by the molluscicidal plants. In addition, some other parameters as glycogen, glucose, total protein, 5-nucleotidase alpha-hydroxybutyrate dehydrogenase [HBDH] and succinate dehydrogenase [SDH] as Krebs cycle enzyme were tested. The study concluded that LC25 and LC50 concentrations of C. spinosa and A. arabica might render B. alexandrina physiologically unsuitable for S. mansoni infection

Influence of capparis spinosa and acacia arabica on certain biochemical haemolymph parameters of Biomphalaria alexandrina

This work investigated the molluscicidal potency of dried Capparis spinosa and Acacia arabica leaves on some selected biochemical parameters of Biomphalaria alexandrina, in order to render them, physiologically, unsuitable for S. mansoni infection or at least disturb the lifecycle of the parasite within its respective snail host. The effect of the two plants on lactate dehydrogenase [LDH], 5'-nucleotidase, acid phosphatase [AP], aspartate and alanine aminotransferases [AST and ALT], alkaline phosphatase [ALP] and glucose content were studied. This work was extended to evaluate the effect of these two plants on protein profile as well as total protein [TP] content of snail's in hemolymph after 24 hours and 1 week of snails plants feeding. The study revealed that both plants induced a marked alteration in all measured parameters, where LC50 of C. spinosa after feeding at one week showed the most potent effect

In vivo, attenuation of Schistosome cercarial development and disturbance of egg laying capacity in Biomphalaria alexandria using sublethal concentrations of plant molluscicides

The dry powder of Sinapis arvensis, Thymelaea hirsuta, Callistemon lanceolatus and Peganum harmala showed molluscicidal activity against Biomphalaria alexandrina, specific intermediate hosts to Schistosoma mansoni. Effect of LC[25] of dry powdered plant molluscicdes on hexokinase [HK], glucose phosphate isomerase [GPI], AMP deaminase, adenosine deaminase and phenol oxidase [PO] of B. alexandrina was traced. C. lanceolatus showed the highest molluscicidal activity as it has the lowest LC[50] compared to S. arvensis, T. hirsuta, and P. harmala, LC[25] of the latter three plants resulted in more significant inhibition of HK, GPI, AMP deaminase and PO than C. lanceolatus. Treatment of snails with LC[10] of these plants markedly affected compatibility of B. alexandrina to S. mansoni infection. Significant decrease in cercarial production was recorded in snails treated with sublethal concentrations of S. arvensis, T. hirsuta, and P. harmala. Remarkable impairment of the egg laying capacity of molluscicide treated snails was also recorded. Correlation between activity levels of HK, GPI and AMP deaminase and compatibility to parasitic infection and role of PO in the egglaying capacity of these snail species were discussed

Susceptibility of biomphalaria Alexandria to infection with schistosoma mansoni: Correlation with the activity of certain glycolytic enzymes

The importance of the glycolytic flux for the success of Biomphalaria- Schistosome sporocyst interaction was acertained in this study. Hexokinase [HK], pyruvate kinase [PK], glucose phosphate isomerase [GPI] and lactate dehydrogenase [LD], as four important glycolytic enzymes, were markedly stimulated in trematode infected Biomphalaria alexandrina when measured two weeks post exposure to infection with Schistosoma mansoni miracidia. Treatment with this plant resulted in a significant inhibition of these three investigated enzymes. LC10 concentrations of S. nigrum reduced considerably the infection rate of B. Alexandrina with S. mansoni to be 34% compared to an infection rate of 80% in control, non-treated snails. Susceptibility of B. alexandrina to infection with the digenetic trematode S. Mansoni is correlated to the activity levels of the glycolytic enzymes. Moreover, sublethal and less pollutant concentration of S. Nigrum could be recommended to control schistosomiasis by disturbing the intramolluscan environment of the parasite